Please contact a technical service representative for more information. This product is for research use only and is not intended for therapeutic or diagnostic applications. Broad reactivity makes this antibody an excellent loading control. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Purity/Specificityīeta Actin Antibody Peroxidase Conjugated antibody is directed against human beta Actin protein. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Beta actin present in neuromuscular junctions also stains. Beta actin present in fibroblast connective tissue stains very brightly. Specific conditions for reactivity should be optimized by the end user. Application Noteīeta Actin Antibody Peroxidase Conjugated antibody has been tested for use in ELISA and is suitable in immunohistochemistry and western blot. Beta-Actin Peroxidase conjugate Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility.
The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified.
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